Experimental Methods for Burkitt Lymphoma Genome Sequencing Project
On this page researchers can find detailed information describing how CGCI data was generated by genomic platform, including protocols for establishing high-quality nucleic acid samples.?The sequencing protocols were performed at British Columbia Cancer (BC Cancer).
CGCI Sample Naming?
CGCI samples are named using a coding system specific to OCG characterization programs. Please use the OCG Sample Codes?document to properly discern the metadata reiterated within the sample name.?OCG Sample Codes_finalized 05 2017.pdf
Nucleic Acid Sample Processing
CGCI project teams use high-quality RNA and DNA from case-matched tumor and normal tissues to generate comprehensive genomics data. Below is a table outlining how the?project team generated those samples.
|General Methodology||Sample Preparation Protocols|
|DNA/RNA AllPrep Kit (Qiagen), mirVana miRNA Isolation Kit (Ambion), High Pure miRNA Kit (Roche), and QiaAmp blood midi kit (Qiagen)||BL|