Accessing CGCI Data
- ANNOUNCEMENT -
The CGCI data matrix?will not function properly in Internet Explorer unless the Compatibility View is completely turned off.
The CGCI Initiative produces large-scale genomic data sets for adult and pediatric cancers. As participants in a?“community resource projectOpens in a New Tab,”?CGCI members are required to share data with the broader research community to facilitate and encourage new discoveries. Participants in the HIV+ Tumor Molecular Characterization Project (HTMCP) and the Burkitt Lymphoma Genome Sequencing Project (BLGSP) must be familiar with the CGCI Publication Guidelines.
7x彩票网邀请码Read the following user guide to learn how to search and download data generated by CGCI.
- About the Data
- Open vs. Controlled Access
- How to Access Protected Data
- About the CGCI Data Matrix
- How to Navigate the CGCI Data Matrix
CGCI researchers use sequencing and, in some cases, array-based methods to find novel genetic alterations in tumors. They analyze high quality tissue samples using one or more of the following genomic approaches:
- Whole Genome Sequencing
- Transcriptome Sequencing?
- Targeted Gene Sequencing?
To learn more about these approaches, visit the CGCI Research page.
CGCI employs stringent human subjects’ protection and data access policies to protect the privacy and confidentiality of the research participants. Depending on the risk of patient identification, CGCI data are?available to the scientific community in two tiers: open- or controlled-access. Both types of data can be accessed through the CGCI Data Matrix.
7x彩票网邀请码 Data within this category present?minimal risk of patient?identification. Much of CGCI data, excluding patient identifiers, are open-access. These types of data can be analyzed, for example, to make correlations between expression of genomic variants in molecular subtypes and clinical outcomes.CGCI provides the scientific community the maximum amount of open-access data allowable under HIPAA guidelines. Access to this data does not require user certification, and researchers may explore data content without restriction.
Researchers can access these?data by clicking on any link labeled "DCC Open" in the CGCI Data Matrix. Data Use Certification (i.e. approval) is?not?required, and researchers may explore data without restriction.
Examples of open-access data are :?
- Clinical information that could not be used to identify the patient
- Tissue pathology data
- Gene expression data (other than 1o exon array data or mRNA-seq)
- Chromosome-specific copy number alterations and loss of heterozygosity
- Sequence data of single amplicons (matched tumor and normal when available; cannot be assembled to link to an individual)
- Tumor-associated (somatic) mutations
Data within this category present?a small but significant risk of patient re-identification. While stripped of direct patient identifiers as defined by HIPAA, controlled-access data contains specific patient/tumor information and unverified or raw molecular data (e.g., array-based and sequencing files). These data can be used to perform sophisticated bioinformatics analyses. Access to this data requires user certification which can be obtained through NCBI’s dbGaP (National Center for Biotechnology Information’s database of Genotypes and Phenotypes).? Researchers apply for access by filling out a Data Access Request form. Read “How to Access Protected Data” below for more information.
Examples of controlled-access data are :
- Specific demographic and clinical data
- Specific genotype or phenotype data for each case
- Information linking all sequence traces to an individual
- Whole genome, exome or transcriptome sequences for an individual case
General Outline of Instructions:
- Obtain Data Use Certification through dbGaP
- Maintain User Accounts for Data Access
- Access Data via the CGCI Data Matrix
- Use HHS credentials (intramural investigator) or?eRA Commons credentials via Globus.org (extramural investigators) to access data stored at NCI. (Approved PIs and designated downloaders will receive an email with data access instructions upon approval.)
- Use?HHS credentials (intramural investigator) or?eRA Commons credentials (extramural investigator)?account to access data stored in NCBI databases
- Get Help If You Have Trouble Accessing Data
1.? Obtain Data Use Certification through dbGaP
All users requesting access to controlled data must:
7x彩票网邀请码Have an eRA Commons account or HHS credentials (for intramural investigator) to submit requests for access. Further information can be found on the NCBI .
Complete the electronic dbGaP Data Access Request (SF 424 (R&R)) form, which requires a brief description of the investigator’s intended use of the data.? To get approved for a Data Use Certification (DUC), the requestors must:
- Agree to restrict their use of the information for biomedical research purposes only.
- Agree not to try to identify and/or contact the patients.
- Submit requests that agree with the Data Use Limitations specific to the desired data’s appropriate consent group. CGCI has datasets that fall within two separate consent groups. Investigators must get a DUC for each consent group to gain access to all CGCI datasets.
CGCI Data Use Certifications
|Consent Group||Types of Data||Data Use Limitations|
|Pediatric Cancer Research||Pediatric Medulloblastoma?||Access to protected pediatric data will be granted solely for those research projects that can only be conducted using pediatric data (i.e., the research objectives cannot be accomplished using data from adults) and that focus on the development of more effective treatments, diagnostic tests, or prognostic markers for childhood cancers.|
|Cancer Research and General Methods||HIV-related cancers (DLBCL, NSCLC and Cervical Cancer) and Burkitt Lymphoma||Use of the data is limited to scientific research relevant to the biology, prevention, treatment, and late complications of cancers and for the development of applications proposing analytical methods, software, and other research tools.|
- Submit the completed SF 424 (R&R) form electronically to dbGAP for consideration of data access approval.
- Upon SF 424 (R&R) form submission, the signing official of the Principal Investigator’s institution will be notified of the submission and asked to certify agreement with the Data Use Limitations stated within the Data Access Request form.
- After the signing official has certified agreement, the SF 424 (R&R) application will be sent to the NCI Data Access Committee (DAC) to review for approval.? The approval review process can take 2-4 weeks.
- Currently, approval in the form of an individual DUC allows the investigator data access to that consent group’s data for one calendar year.
- Submit a progress report to the DAC no later than one year after obtaining the DUC. The requestor needs to understand that a progress report is a current condition for the data access. Approved users may also apply for renewal to access protected data at the same time they submit the reports. A reminder to submit an annual progress report and renew approval status, if needed, will be sent by the DAC staff approximately one month before the access termination deadline. If the requestor does not submit the progress report or requests a renewal, access to the data will cease.
2.? Maintain User Accounts for Data Access
7x彩票网邀请码Intramural investigators with an approved DUC may access protected CGCI data using their HHS credentials.
Investigators outside of HHS with an approved DUC require two separate user accounts to access protected CGCI data:
- For access to CGCI data stored and maintained at NCBI – approved users can access CGCI data stored at NCBI using the eRA Commons account associated with the original Data Access Request. CGCI data stored at NCBI includes Sanger sequencing files and aligned reads from 2nd generation sequencing (BAM files).
- For access to data stored and maintained at the OCG Data Coordinating Center (DCC) at the National Cancer Institute (NCI) – ?approved users outside of HHS are required to use eRA Commons account credentials to log on to Globus.org in order to access CGCI controlled-access data housed at the National Cancer Institute’s Office of Cancer Genomics Data Coordinating Center (NCI OCG DCC). (Approved PIs and designated downloaders will receive an email with detailed instructions on how to use Globus.org to access OCG DCC data upon approval.)?This account will be used to access data at the OCG DCC, which includes most of the genomic data generated for the CGCI initiative (clinical information, all levels of chip-based molecular characterization, and higher level sequencing data).?***The password on this account needs to be updated every 120 days, and instructions are distributed when the account is created***?
3.? Access Protected Data via the CGCI Data Matrix
Approved users may access protected CGCI data through the CGCI Data Matrix?with either HHS credentials or with eRA Commons credentials via Globus.org (as outlined in #2).
7x彩票网邀请码A Globus.org account associated with eRA Commons is required to access controlled-access CGCI data. (If users already have another non-eRA Commons-related Globus account set up, that should not be a problem, but only eRA-commons Globus accounts can be used to access this dataset.)
- Access data stored at the OCG DCC directly through the CGCI Data Matrix?(requires NCI-issued account for extramural investigators):
- Protected clinical information
- All levels chip-based molecular characterization data
- Processed sequencing data (upper level files, excluding BAM files)
- Access low-level sequence files stored at NCBI indirectly through hyperlinks on the CGCI Data Matrices (requires eRA Commons account for extramural investigators):
- Trace sequences stored in the NCBI TRACE Archives
Sanger targeted sequencing
- BAM files stored in the Sequence Read Archives accessible through NCBI dbGaP
2nd/3rd generation whole genome, exome, mRNA-seq, miRNA-seq
- Trace sequences stored in the NCBI TRACE Archives
4.? Get Help If You Have Trouble Accessing Data
For NCI-stored data – OCG@mail.nih.gov
For NCBI-stored data –
CGCI data is accessible through tabular, easy-to-use Data Matrices. Each project within CGCI has its own Data Matrix. In addition to the individual project-specific data matrices, there is one composite CGCI Data Matrix that provides links to data from all CGCI projects.? New data from ongoing projects gets incorporated into the Data Matrices as it becomes available, along with an update of the matrix version history. Users should note the version of the CGCI Data Matrix when accessing information.
The CGCI Data Matrix evolves over time to meet the needs of the research community. We encourage users to send comments, questions, and suggestions for improvement to firstname.lastname@example.org.
The Data Matrices link to both open and controlled access CGCI data. To obtain specific datasets or metadata, including descriptions of each project, users can hover over the text within the table and click to access the appropriate files.
Raw or low level data files (level 1)
Normalized and integrated data (levels 2 and 3)
Summarized findings (level 4)
Data Access Code
Blue = open access
Red = controlled access (NCI & NCBI)
Black = unavailable
Types of Data Found in the Matrix
Names of diseases studied
Clinical information, including outcomes
Types of molecular data generated and platforms used
Metadata descriptions about each individual project
Multi-level chip-based and sequencing data links