TARGET Project Experimental Methods
7x彩票网邀请码On this page researchers can find detailed information describing how TARGET data was generated by genomic platform, including protocols for establishing high-quality nucleic acid samples.?
*Note to users: Protocols are currently being?added to this site, as a large portion of the data and metadata were recently released onto the TARGET Data Matrix. Please continue to check back in the coming weeks as updates are completed, and?contact firstname.lastname@example.org with any questions.?
TARGET Sample Naming?
TARGET samples are named using a coding system specific to OCG characterization programs. Please use the OCG Sample Codes?document to properly discern the metadata reiterated within the sample name.?OCG Sample Codes_finalized 05 2017.pdf
Nucleic Acid Sample Processing
7x彩票网邀请码TARGET project teams use high-quality RNA and DNA from case-matched tumor and normal tissues to generate comprehensive genomics data. Below is a table outlining how each project team generated those samples.
|General Methodology||Sample Preparation Protocols|
|DNA/RNA Co-isolation with Qiagen AllPrep Kit (DNA/RNA) and mirVana (Total & Small RNAs)||AML , AML-IF , CCSK , RT , WT , OS , NBL (after 2013)|
|DNA Isolation with Qiagen QIAamp DNA Minikit||ALL P1 , ALL P2 , ALL MDLS|
|DNA Isolation with Qiagen Genomic-Tips||NBL (prior to 2013) , WT|
|RNA Isolation with Invitrogen TRIzol||ALL P1 , ALL P2 , ALL MDLS|
|RNA Isolation with Invitrogen TRIzol and Qiagen RNAeasy||NBL (prior to 2013)|